Composite
REDMAP-GOI

Part:BBa_K4211014:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-09-30)


REDMAP-GOI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of interest (GOI) is between 5×UAS-P(hCMVmin) (BBa_K4211005) and CMV promoter (BBa_K1439007). The users can insert the target gene into this position. Of course, we suggest that the users should add a terminal sequence like TAA or TGA at the C-terminal of the target gene.


Source

It was designed by the BNUZH-China iGEM team 2022 based on the article in Nature Biotechnology: A small and highly sensitive red/far-red optogenetic switch for applications in mammals.

References

Zhou, Y., Kong, D., Wang, X. et al. A small and highly sensitive red/far-red optogenetic switch for applications in mammals. Nat Biotechnol 40, 262–272 (2022). https://doi.org/10.1038/s41587-021-01036-w